Epigenomic signatures of sarcomatoid differentiation to guide the treatment of renal cell carcinoma

Renal cell carcinoma with sarcomatoid differentiation is associated with poor survival and heightened response to immune checkpoint inhibitors. Two major barriers to improving outcomes for sRCC are the limited understanding of its gene regulatory programs and the low diagnostic yield of tumor biopsies due to spatial heterogeneity. Chromatin immunoprecipitation DNA-sequencing (ChIP-seq) for histone modifications H3K27ac, H3K4me2 on tissue samples. Methylated immunoprecipitation and high-throughput sequencing (MeDIP-seq) on plasma and tissue samples.