Enhancer analysis of rat cardiac myocytes and fibroblasts reveals a collaborative control by transcription factor families [ChIP-Seq]

We used a differential open chromatin approach, coupled with active enhancer mark ,transcriptomic and computational TFs binding analysis to map cell-type-specific active enhancers in cardiac fibroblasts and cardiomyocytes, and outline the TFs that control them. We identified Tead, Sox9, Smad, Tcf, Meis, Rbpj, and Runx1 as the main cardiac fibroblasts TF groups. Our analysis shows that in cardiomyocytes and cardiac fibroblasts, distal enhancers, containing concentrated combinatorial clusters of multiple tissue expressed TFs recognition motifs, are combinatorically clustered around tissue specific genes. Overall design: We examined 3 different biological samples ('cardioPE', 'cardioSFM' (cardiomyocytes in different conditions) and 'cardiacFibro'), in duplicates, to identify histone modification by using ChIP-seq assay.