Homo sapiens isolate:SW480 Raw sequence reads

Genetic screens using CRISPR/Cas9 are a powerful method for the functional analysis of genomes. Here we describe CRISPR library designer (CLD), an integrated bioinformatics application for the design of custom single guide (sg) RNA libraries for all organisms with annotated genomes. CLD is suitable for the design of libraries using modified CRISPR enzymes and targeting non-coding regions. To demonstrate its utility, we performed a pooled screen for modulators of the TNF-related apoptosis-inducing ligand (TRAIL) pathway using a custom library of 12,471 sgRNAs. We show that CLD predicts a high fraction of functional sgRNAs. CLD is publicly available at https://github.com/boutroslab/cld.