Untargeted LC-HRMS-based metabolomic profiling reveals distinct metabolomic profiles in patients with metabolic dysfunction-associated steatotic liver disease (MASLD)

We analyzed liver tissues, liver secretome (conditioned medium), and blood plasma samples from 109 obese patients undergoing bariatric surgery spanning early stages of metabolic dysfunction-associated steatotic liver diseases (MASLD). We used a combination of data-dependent acquisition (DDA) and data-independent acquisition (DIA or SWATH-MS) on SCIEX TripleTOF 6600,

Metabolites and lipids were extracted using modified chloroform-methanol biphasic extraction methods for both biological matrices. Organic and aqueous fractions were separated using RRHD Eclipse Plus C18 and SeQuant ZIC-cHILIC columns prior to DDA and DIA acquisitions, respectively.

All mzML files are deposited with ZENODO.

*** DDA files are deposited in this dataset repository ***

Child directories for SWATH data files

Samples
Polarity
Zenodo DOI

Organic fraction - Reverse Phase
Positive & Negative

10.5281/zenodo.14096635

Aqueous fraction - HILIC
Positive

10.5281/zenodo.14096753

Aqueous fraction - HILIC
Negative

10.5281/zenodo.14136832