Sirtuins may mediate temperature-induced metabolic remodeling in threespine stickleback

The molecular pathway governing metabolic remodeling in response to temperature in fishes is unknown. We tested the hypothesis that sirtuins, a family of NAD+ dependent lysine deacetylases, drives an increase in aerobic metabolism in response to cold acclimation in stickleback. Stickleback were acclimated to 5, 12 and 20°C and sirtuin activity measured in liver, oxidative and glycolytic muscles using a Promega SIRT-Glo Assay kit. Protein levels of SIRT1 and SIRT3 were quantified using custom antibodies and capillary western blotting. In a second set of experiments, sirtuin activity was inhibited by feeding with EX-527 or activated by feeding with the NAD+ pre-cursor, nicotinamide mononucleotide (NMN). Standard metabolic rate (SMR) was measured using intermittent flow respirometry. Mitochondrial respiration rates were measured in permeabilized liver tissue using a substrate-uncoupler-inhibitor titration protocol . Sirtuin activity significantly increased in response to cold acclimation i..., , # Sirtuins may mediate temperature-induced metabolic remodeling in threespine stickleback

Dataset DOI: [10.5061/dryad.n8pk0p38d](https://doi.org/10.5061/dryad.n8pk0p38d)

## Description of the data and file structure

Stickleback (*Gasterosteus aculeatus*) were acclimated to 5, 12, and 20°C for 13 weeks. Sirtuin activity was then measured in liver, oxidative, and glycolytic muscles using a Promega SIRT-Glo assay kit, and protein levels of SIRT1 and SIRT3 were quantified using custom antibodies and capillary western blotting. In a second set of experiments, sticklebacks were acclimated to 20°C for 12 weeks. A subset of fish were then moved into an environmental chamber where the temperature in the room was decreased by 3°C per day over 2 days and then by 4°C on day 3 to reach 10°C. After 14 days at 10ºC, bloodworms were supplemented with either the sirtuin inhibitor EX-527 or the sirtuin activator, NMN, mixed with 7% agar for 14 days. Standard metabolic rate was then measured using inte...,