Analysis of chromatin occupancy in erythroid cells by CUT&RUN following CRISPR editing of BCL11A enhancer

The mechanisms underlying the remarkable efficacy of CRISPR-mediated BCL11A enhancer ablation remain poorly understood. Here, we employ CUT&RUN to investigate active and repressive histone modifications, as well as cohesin and CTCF binding, to uncover epigenetic changes under various editing conditions. Overall design: The CUT&RUN assay was performed following enhancer knockout or sg1617 editing to assess chromatin alterations. The inactive chromatin mark H3K27me3, as well as the occupancy of cohesin, and CTCF, were examined in wild-type cells, single-cell clones with different INDELs, and bulk-edited CD34+ HSPCs.