Electrically stimulated hiPSC-derived astrocytes - calcium imaging videos, raw and analysed fluorescence intensity values

Deep brain stimulation (DBS) is an established neuromodulation technique used for various conditions. While it offers symptom relief, the mechanisms underlining its therapeutic effects remain unclear, affecting efficacy and side effects. A key challenge in DBS research is isolating neuron-astrocyte interactions in a complex in vivo environment. To address this, we developed tools to study the effects of DBS on astrocytic calcium dynamics in vitro. We employed human induced pluripotent stem cell (hiPSC)-derived astrocytes cultured on custom glass micro-electrode arrays (MEAs) together with a semi-automated analysis pipeline to extract key calcium dynamics parameters of large data sets. Here, we share calcium imaging videos of hiPSC-derived astrocytes stimulated at 10Hz, 130Hz and stimulated with one electrical pulse. Additionally, we provide the raw fluorescence intensity values of Fluo-4 Calcium indicator in each identified cell and the subsequent data analysed using our custom-made Fluorescence analyser script.