Consensus sequence and mutant spectrum composition of the 3D-coding region of FMDV R-Ap35 and R-Ap60, and deduced amino acid substitutions.

aMutations found in the consensus sequence at the 3D-coding region of populations R-Ap35 and R-Ap60, relative to the genomic sequence of the parental FMDV MARLS (Figure 1). Two nucleotides separated by a dash indicate the presence of a mixture, as judged from the nucleotide sequence chromatogram. FMDV genomic residues are numbered as previously described [93]. Procedures for nucleotide sequencing are described in Materials and Methods.
bAmino acid substitutions in 3D that result from the mutations found in R-Ap35 or R-Ap60 populations indicated in the first two columns. Replacements that are totally imposed in R-Ap35 or R-Ap60 are underlined. “ = ”means a synonymous mutation.
cFrequency of the amino acid that results form each mutation, as indicated in the third column (S44, V132, S169, K282, I296) found in two or more molecular clones from the corresponding mutant spectrum. Mutations and deduced amino acid substitutions are based on the analysis of 18,620 nucleotides of the mutant spectrum of the 3D-coding region from population R-Ap35 (14 clones), and 19,950 nucleotides in the case of R-Ap60 (15 clones). Procedures for mutant spectrum analysis are described in Materials and Methods.