Salivary gland transcriptomic analysis in the IL-14α transgenic mouse model of Sjögren’s disease using Visium V1 3' spatial gene expression assay

Sjögren’s disease (SjD) is a systemic autoimmune disorder primarily affecting the exocrine glands and characterized by dry mouth and dry eye, the presence of anti-SSA and/or anti-SSB autoantibodies in blood serum, and chronic lymphocytic infiltration of salivary and lacrimal glands (i.e., sialadenitis and dacryoadenitis, respectively). The interleukin-14α transgenic (IL-14αTG) mouse model of SjD recapitulates many aspects of human SjD, including progressive sialadenitis, loss of salivary gland function, and development of B cell lymphoma. We performed Visium V1 3' spatial transcriptomic analysis of fresh frozen submandibular and sublingual glands from a 12-month-old IL-14αTG Sjögren’s-like mouse and age-matched C57BL/6 control mouse. One submandibular gland and one sublingual gland from 12-month-old female IL-14α transgenic or 12-month-old C57BL/6 control mouse was included in the Visium capture area for spatial transcriptomic analysis.