Gene expression changes in Nipbl+/− mouse limb buds.

Relative gene expression levels in limb buds of stage-matched, E10.5 wildtype and Nipbl+/− mice were determined as described (see Materials and Methods), and in certain cases confirmed by Q-RT-PCR. Selected transcripts are shown.iInvolved in control of Shh expression in limb bud [101].iiAlso controls Hox gene expression in the limb bud [102].iiiAlso controls Hox gene expression in the limb bud [69].ivDirect target of Hoxd13 in limb buds [103].vDirects the position of the Shh expression boundary delineating the experimentally defined ZPA [104].viMesenchymal, involved in chondrocyte proliferation [105].viiAER-Fgf[106].viiiStrongly activated by HOXA13 [67].ixHOXA13 target in limb buds [68].xFunctions as a HOX cofactor during development; complexes with HOXA9; also controls Hox and Shh expression [102].xiNon-canonical Wnt [107].xiiCanonical Wnt [108].xiiiInteracts with some Wnts and Frizzleds and supports Wnt-Fz-Ror2 complex formation, and at the same reduces Wnt-Fz-LRP complex formation, thus favoring non-canonical Wnt signaling [109].xivWnt regulator; required for maintenance of AER and Shh signaling [110].Gene expression changes in Nipbl+/− mouse limb buds.