Effect of rivaroxaban on the collagen-stimulated PDGF-AB secretion from human platelets.

PRP was pretreated with 1000 ng/ml of rivaroxaban or vehicle for 15 min, and then stimulated by 1.0 μg/ml collagen or vehicle for 5 min. The reaction was terminated by the addition of ice-cold EDTA (10 mM) solution. The conditional mixture was centrifuged at 10,000 x g at 4°C and the supernatants were then subjected to an ELISA for PDGF-AB. Values for unstimulated platelets have been subtracted from each data point. Results from ten healthy donors are shown. Each value represents the mean ± SEM. N.S. designates no significant difference between the indicated pairs.