肺癌T细胞研究

本研究对来自14个药物治疗前非小细胞肺癌患者的外周血、癌旁组织和癌组织的12,346个T细胞进行了单细胞转录组测序，全面描绘和解析了肺癌T细胞群体的组成、谱系以及功能状态图谱。基于T细胞的转录组数据及TCR序列，通过生物信息学手段分析细胞的亚群分类、组织分布特征、肿瘤内群体异质性及药物靶基因表达情况，该研究鉴定了跨组织分布的T细胞类群、肿瘤浸润T细胞的组成及亚群间潜在的状态转换关系，提出了新的肺腺癌临床标志物，也为免疫治疗特异性地靶向T细胞亚群提供了新的思路。In this study, single-cell RNA sequencing was performed on 12,346 T cells isolated from peripheral blood, adjacent normal tissues, and tumor tissues of 14 drug-naïve patients with non-small cell lung cancer (NSCLC). This research comprehensively depicts and dissects the landscape of the composition, lineage, and functional status of T cell populations in lung cancer. Based on the transcriptomic data and T cell receptor (TCR) sequences of these T cells, bioinformatic analyses were conducted to investigate cell subpopulation classification, tissue distribution characteristics, intratumoral population heterogeneity, and the expression patterns of drug target genes. Furthermore, this study identified T cell subsets with cross-tissue distribution, the composition of tumor-infiltrating T lymphocytes (TILs), and potential state transition relationships among subpopulations, proposed novel clinical biomarkers for lung adenocarcinoma, and provided new insights into the specific targeting of T cell subsets for cancer immunotherapy.