Secondary structure of Zn(II)Har and Zn(II)Har150 in the absence or presence of Fe2+ or hemin.
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Secondary structures were estimated by DichroWeb [33], [34] analyses of the CD data of the proteins (5 µM) at 20°C in the absence or presence of Fe2+ (1 eq) and hemin (0.8 eq) in 10 mM HEPES reducing buffer (75 mM NaCl, 0.5 mM TCEP, pH 7.0) and 10 mM PBS (75 mM NaCl, pH 7.4), respectively.Secondary structure of Zn(II)Har and Zn(II)Har150 in the absence or presence of Fe2+ or hemin.
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2015-12-02



