X-ray Structure of the H225N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product

X-ray Structure of the H225N mutant of TcaB9, a C-3'-Methyltransferase, in Complex with S-Adenosyl-L-Homocysteine and Sugar Product Descriptor: (2R,4S,6R)-4-amino-4,6-dimethyl-5-oxotetrahydro-2H-pyran-2-yl [(2R,3S,5R)-3-hydroxy-5-(5-methyl-2,4-dioxo-3,4-dihydropyrimidin-1(2H)-yl)tetrahydrofuran-2-yl]methyl dihydrogen diphosphate (non-preferred name), 1,2-ETHANEDIOL, S-ADENOSYL-L-HOMOCYSTEINE, ... Authors: Bruender, N.A, Holden, H.M. Deposit date: 2012-03-09 Release date: 2012-04-25 Last modified: 2023-09-13 Method: X-RAY DIFFRACTION (1.41 Å) Cite: Probing the catalytic mechanism of a C-3'-methyltransferase involved in the biosynthesis of D-tetronitrose. Protein Sci., 21, 2012

TcaB9的H225N突变体的X射线晶体结构，该蛋白属于C-3'-甲基转移酶（C-3'-Methyltransferase），与S-腺苷-L-同型半胱氨酸（S-Adenosyl-L-Homocysteine）及糖产物形成复合物。糖产物标识符为：(2R,4S,6R)-4-氨基-4,6-二甲基-5-氧代四氢-2H-吡喃-2-基 [(2R,3S,5R)-3-羟基-5-(5-甲基-2,4-二氧代-3,4-二氢嘧啶-1(2H)-基)四氢呋喃-2-基]甲基二氢二磷酸（非惯用名称）、1,2-乙二醇（1,2-ETHANEDIOL）、S-腺苷-L-同型半胱氨酸…… 作者：Bruender, N.A.、Holden, H.M.。沉积日期：2012-03-09，发布日期：2012-04-25，最后修改日期：2023-09-13。实验方法：X射线衍射（分辨率1.41 Å）。引用文献：《探究参与D-四硝糖生物合成的C-3'-甲基转移酶的催化机制》，《Protein Sci.》，21卷，2012年。