RNA seq of pancreatic cancer-associated fibroblasts

Transcriptomic profiling of pancreatic cancer-associated fibroblasts (CAFs) was done to evaluate the expression of GPCRs. The same was also done for normal pancreatic stellate cells, so as to evaluate whether a population of GPCRs shows increased expression in CAFs vs their normal precursors. We report the discovery of a novel GPCR in CAFs; we demonstrate this GPCR helps to drive the cross-talk between CAFs and cancer cells, enhancing the growth of pancreatic cancer cells. Stranded mRNA sequencing of 3 lines of de-identified patient-derived, low-passage pancreatic CAFs and 1 line of normal pancreatic stellate cells (PSCs)