Confocal image stacks of GFP expression in Drosophila forelegs driven by Gal4 driver expression in foreleg motor neurons

Like the vertebrate spinal cord, the insect ventral nerve cord (VNC) mediates limb sensation and motor control. We applied automated tools for electron microscopy volume alignment, neuron segmentation, and synapse prediction toward creating a connectome of an adult female Drosophila VNC. To interpret a connectome, it is crucial to know its relationship with the rest of the body. We therefore mapped the muscle targets of leg and wing motor neurons in the connectome by comparing their morphology to genetic driver lines, dye fills, and X-ray nano-tomography of the fly leg and wing. The dataset made available here contains the confocal image stacks of the legs of each driver line. We imaged only driver lines for which we found an image of a single motor neuron labeled by MCFO, from the collection on the https://neuronbridge.janelia.org website. Thus, the dendritic morphology of the MNs that are visible in these images stacks can be found by cross referencing the alphanumeric code for the Gal4 driver line.