Confocal image stacks of GFP expression in Drosophila forelegs driven by Gal4 driver expression in foreleg motor neurons

Like the vertebrate spinal cord, the insect ventral nerve cord (VNC) mediates limb sensation and motor control. We applied automated tools for electron microscopy volume alignment, neuron segmentation, and synapse prediction toward creating a connectome of an adult female Drosophila VNC. To interpret a connectome, it is crucial to know its relationship with the rest of the body. We therefore mapped the muscle targets of leg and wing motor neurons in the connectome by comparing their morphology to genetic driver lines, dye fills, and X-ray nano-tomography of the fly leg and wing. The dataset made available here contains the confocal image stacks of the legs of each driver line. We imaged only driver lines for which we found an image of a single motor neuron labeled by MCFO, from the collection on the https://neuronbridge.janelia.org website. Thus, the dendritic morphology of the MNs that are visible in these images stacks can be found by cross referencing the alphanumeric code for the Gal4 driver line.与脊椎动物的脊髓功能相似，昆虫腹神经索（ventral nerve cord, VNC）介导肢体感知与运动调控。本研究借助自动化工具完成电子显微镜体积配准、神经元分割与突触预测工作，旨在构建成年雌性果蝇腹神经索的连接组（connectome）。若要解析该连接组，明确其与生物体其余部分的关联至关重要。为此，我们将该连接组内腿部与翅膀运动神经元（motor neurons）的形态，与遗传驱动系、染料填充标记以及果蝇腿部和翅膀的X射线纳米断层扫描结果进行比对，从而绘制出这些运动神经元的肌肉靶标分布。本文公开的数据集包含各遗传驱动系腿部的共聚焦图像堆栈（confocal image stacks）。本次成像仅针对那些可在https://neuronbridge.janelia.org网站的藏品库中获取到经MCFO标记的单个运动神经元图像的遗传驱动系。因此，通过检索该Gal4驱动系的字母数字编码，即可获取这些图像堆栈中可见的运动神经元树突形态信息。