Leaf and root transcriptome sequencing of tomato accessions

RNAseq was performed on leaf and root tissue from two accessions of tomato to identify NLRs and their relative expression level. Leaves and roots were sampled at one month post germination. Leaf tissue was collected from all fully expanded leaves, whereas roots were sampled and washed in distilled water. Accessions were sequenced using 150 bp paired end reads. RNAseq read quality was assessed and trimmed using Trimmomatic (version 0.36) with the parameters ILLUMINACLIP:2:30:10, LEADING:3, TRAILING:3, SLIDINGWINDOW:4:15, and MINLEN:100. Transcriptome assembly was performed using Trinity (version 2013-11-10) with default parameters.