pfkA encodes a functional phosphofructokinase.

Fructose-6-phosphate kinase activity of cell-free extracts from M. tuberculosis strains was measured by coupling fructose-1,6-bisphosphate formation to oxidation of NADH with aldose, triosephosphate isomerase and α-glycerophosphate dehydrogenase. Each biological sample was measured in duplicate. The data represent the values obtained for each duplicate of each biological sample. ∧ Enzymatic assay of purified recombinant His-tagged PFKA and His-tagged PFKB of M. tuberculosis was performed in triplicates and results are expressed as mean ± SD. Each experiment was repeated as least once independently and comparable values and trends were observed. Legend: nd, not detectable.