Ribosomal protein S6 phospho-deficient mice display altered novelty-induced locomotion, synaptic plasticity, and specific mRNA translation. Mus musculus

By high-throughput RNA-seq of heavy polysomal fractions, we report that rpS6 knockin mice, in which the 5 phosphorylatable serines have been replaced by alanines, show an alteration in the translation, but not transcription, of a subset of mRNAs in the nucleus accumbens (NAc) but not in the dorsal striatum (DStr). Gene Ontology analysis revealed that multiple dysregulated mRNAs are related to the mitochondria. Moreover, we described that rpS6 knockin mice also display an alteration in novelty-induced locomotion and synaptic plasticity. Overall design: mRNAs from heavy polysomal fractions (Heavy) and total RNA (Total) from both the nucleus accumbens (Nac) and dorsal striatum (DStr) were compared between rpS6 knockin mice and wildtype littermates. We sequenced triplicates per genotype and brain region.