Differential expression analyses in microglial cells from APP-Psen1/+;Cx3cr1-Cre::ERT2/+; Vhl Flox/– mice treated with tamoxifen vs APP-Psen1/+;Cx3cr1-Cre::ERT2/+; Vhl Flox/– mice non treated

Microglial cells were isolated by flow citometry using specific markers (CD45+/ CD11b+) and their global gene expression profile was analyzed. This data were used to study the role of HIF1A in microglia associated to Aβ plaques. In this dataset, we include the expression data obtained in microglial cells from a model of gain of function of HIF1A in APP-PSEN1/+ mice. (18 moth-old-age) mice model using a specific marker for this cell type (CD31+). Functional enrichment analyses were carried out through GSEA, where we have included specific categories potentially involved with blood vessel formation. 6 Total samples were analyzed with 3 biological replicates in each condition (APP-PSEN1/+;Cx3cr1-Cre::ERT2/+; Vhl Flox/– treated with tamoxifen vs the same non treated genetic mouse model). We generated the following comparison: treated vs non treated APP-PSEN1/+; Cx3cr1-Cre::ERT2/+; Vhl Flox/–). Data were normalized by using rma algorithm (robust multiarray average) and the normalized matrix that we obtained with this analyses was used such input in the GSEA tool.