SulfAtlas

Widespread in nature, sulfated biomolecules are highly diverse in chemical structure and biological function. These compounds include sulfate esters (ROSO3-) and sulfamates (RN(H)SO3-) and range from small molecules to complex polymers. Sulfatases cleave sulfate groups from such molecules and constitute a biologically and industrially important group of enzymes. However, the number of sulfatases which have been characterized is limited in comparison to the huge diversity of the sulfated compounds. In the context of the explosion of genomic data, the functional annotation of sulfatases is thus particularly prone to flaws and misinterpretations. A classification system allowing for a better prediction of substrate specificity and for setting the limit of functional annotations was therefore urgently needed for sulfatases. SulfAtlas describes the family and sub-families of structurally-related sulfatases. Sub-families are created based on phylogenetic analyses and essentially correspond to different substrate specificities. Experimental evidences for the activity of new sulfatases will be regularly updated.硫酸化生物分子（sulfated biomolecules）在自然界中分布广泛，其化学结构与生物学功能均具有高度多样性。该类化合物涵盖硫酸酯（sulfate esters，ROSO₃⁻）与氨基磺酸盐（sulfamates，RN(H)SO₃⁻）两类，分子量跨度从小分子直至复杂聚合物。硫酸酯酶（sulfatases）可催化此类分子脱去硫酸基团，是一类在生物学与工业领域均具有重要价值的酶类。然而，相较于硫酸化化合物的庞大多样性，已完成功能表征的硫酸酯酶数量仍十分有限。在基因组数据爆发式增长的当下，硫酸酯酶的功能注释极易出现偏差与误判。因此，亟需一套分类系统以实现对底物特异性的精准预测，并为功能注释划定合理边界。SulfAtlas收录了结构相关的硫酸酯酶家族与亚家族信息：亚家族基于系统发育分析构建，本质上对应不同的底物特异性；新发现的硫酸酯酶活性实验证据将得到定期更新维护。

搜集汇总

背景与挑战

背景概述

SulfAtlas是一个硫酸酯酶数据库，旨在通过基于系统发育分析的分类系统，解决硫酸酯酶功能注释中的缺陷，以更好地预测底物特异性。该数据库描述了硫酸酯酶家族和亚家族，并会定期更新新酶的实验证据。