Transcriptional regulation in denervated murine GA muscle. Mus musculus

Samples of denervated GA muscle were compared to contralateral controls to determine transcriptional changes associated with skeletal muscle denervation. Overall design: Denervation was performed as described (Moresi et al., 2010). In brief, adult mice were anesthetized with isoflurane. The sciatic nerve of the left leg was exposed and a 2 mm piece was removed. The right leg remained innervated and was used as a control. Mice were sacrificed and the gastrocnemius muscles was then prepared and snap frozen in liquid nitrogen 14 days after denervation.