RNA-Seq analysis of kidney from female 5/6Nx B6 mice and female sham B6 mice

Aim We have reported that D-Ser and D-Ala are protective against renal injury in acute kidney injury model mice. We generated 5/6-Nx-induced chronic kidney disease model mice with more severe renal injury and evaluated the protective effects of D-Ser and D-Ala. Methods Mice WT C57BL/6 mice were purchased from CLEA Japan (Osaka, Japan). All mice were housed and bred at Kanazawa University. D-Ser and D-Ala (Wako, Tokyo, Japan) was administrated orally at a concentration of 20 mM in distilled water. Mice were allowed free access to D-Ala dissolved water. 5/6-Nx Induction Progressive renal failure was induced via 5/6-Nx in a two-step procedure using 6-8 weeks mice. For initial 2/3 nephrectomy, the left kidney was exposed via flank incision. Clamp the renal arteries and veins, Upper and lower kidney poles were cut using bipolar. Furthermore, liquid thrombin (MOCHIDA PHARMACEUTICAL CO., LTD. Tokyo, Japan) was attached to the cut surface to stop bleeding. Right kidney nephrectomy was carried out after two weeks of recovery. Removal of the second kidney represents day 0. Sham surgery was also performed as a two-step procedure in which the respective kidney was exposed and then repositioned. The kidney samples enrolled in this study were treated with D-Ser and D-Ala and non-treated mice.