Mice heterozygous for each of 3 independent Surf4 targeted alleles are observed at expected Mendelian ratios at weaning.

Mice heterozygous for the indicated Surf4 allele were crossed with wild-type C57BL/6J mice and the resulting litters genotyped for the corresponding Surf4 alleles at approximately 2 weeks of age. The proportion of mice with the heterozygous mutant genotype was compared to expected Mendelian ratios by the chi-square test.