Data from: Specific 50-kHz vocalizations are tightly linked to particular types of behavior in juvenile rats anticipating play

AbstractRat ultrasonic vocalizations have been suggested to be either a byproduct of physical movement or, in the case of 50-kHz calls, a means to communicate positive affect. Yet there are up to 14 distinct types of 50-kHz calls, raising issues for both explanations. To discriminate between these theories and address the purpose for the numerous 50-kHz call types, we studied single juvenile rats that were waiting to play with a partner, a situation associated with a high number of 50-kHz calls. We used a Monte-Carlo shuffling procedure to identify vocalization-behavior correlations that were statistically different from chance. We found that certain call types (“split”, “composite” and “multi-step”) were strongly associated with running and jumping while other call types (those involving “trills”) were more common during slower movements. Further, non-locomotor states such as resting and rearing were strongly predictive of a lack of vocalizations. We also found that the various sub-types of USVs can be clustered into 3–4 categories based on similarities in the way they are used. We did not find a one-to-one relationship between any movements and specific vocalizations, casting doubt on the motion byproduct theory. On the other hand, the use of specific calls during specific behaviors is problematic for the affect communication hypothesis. Based on our results, we suggest that ultrasonic calls may serve to coordinate moment-to-moment social interactions.
Usage notesVocalization Data - Testing Day 7 OnlyThese vocalization files are from the second set of data analysis where all data was taken from the last day (day 7) of data analysis. The files are named R_D_C. R for rat number, D for day of testing and C for the cohort the animal belonged to. These files match the videos uploaded.Vocalization Data - Assorted DaysThese vocalization files are from the first set of data analysis where all data was taken from assorted days of play anticipation and used for data analysis. The files are named R_D_C. R for rat number, D for day of testing and C for the cohort the animal belonged to. These files match the videos uploaded.Behavioural Data -Day 7 OnlyThese video files are from the second set of data analysis where all data of anticipation of play was taken from the last day (day 7) for data analysis. The files are named R_D_C. R for rat number, D for day of testing and C for the cohort the animal belonged to. These files match the video files uploaded.Behavioural Data - Assorted DaysThese vocalization files are from the first set of data analysis where the data was taken from assorted days of anticipation of play for data analysis. The files are named R_D_C. R for rat number, D for day of testing and C for the cohort the animal belonged to. These files match the corresponding vocalization files uploaded.Data for Paper Publication - Video (2).zip