Interactive effects of immediate and ancestral salt stress on fitness in duckweed

Premise of Research Organisms that reproduce asexually must respond to abiotic stresses in their environment while contending with the potential disadvantage of the reduced ability for genetic variation through recombination. Common duckweed (Lemna minor) is an aquatic plant that reproduces predominantly asexually through the budding of ramets. As a freshwater plant, duckweed experiences stress from salt (e.g., NaCl), which detrimentally affects growth, photosynthesis, and cellular processes. We took a demographic approach to examine whether ancestral exposure to salt stress affects the ability of duckweed offspring to tolerate immediate exposure to the same stress. Methodology We placed three consecutive clonal generations of duckweed in an environment with 2 g L-1 NaCl (non-lethal salt stress) or 0 g L-1 NaCl (control), followed by an additional 0-3 generations in the control environment to vary the presence and schedule of ancestral stress. After these treatments, we used the offspri..., , R software is required to run the script.,研究前提 进行无性繁殖的生物，在应对环境中非生物胁迫（abiotic stresses）的同时，还需面对因重组介导的遗传变异能力下降所带来的潜在劣势。普通浮萍（Common duckweed, Lemna minor）是一种水生植物，主要通过分株（ramets）出芽的方式进行无性繁殖。作为淡水植物，浮萍会遭受盐胁迫（如氯化钠NaCl），该胁迫会对其生长、光合作用及细胞生理过程产生不利影响。本研究采用种群统计学方法（demographic approach），旨在探究祖先代暴露于盐胁迫环境是否会影响浮萍子代对同种胁迫的即时耐受能力。 研究方法 我们将连续三代克隆浮萍分别培养于含2 g·L⁻¹ 氯化钠（非致死盐胁迫）或0 g·L⁻¹ 氯化钠（对照组（control））的环境中，随后在对照环境中继续培养0至3代，以此调控祖先代胁迫的施加与否及时序安排。完成上述处理后，我们使用子代[原文此处存在截断]，运行该脚本需依赖R软件。