Ilya Ravkin (2011) CIL:32062, Homo sapiens, osteocarcenoma. CIL. Dataset

This image set is of Transfluor assay where an orphan GPCR (G-protein coupled receptor) is stably integrated into a beta-arrestin GFP (green) expressing U2OS cell line also showing nucleus (red). After one hour incubation with a compound, the cells were quenched with fixative (formaldehyde) and the plate was read on Cellomics ArrayScan HCS Reader using the GPCR Bioapplication. Negative results are a more uniform distribution of GFP throughout the cytoplasm and positive results are a peri-nuclear localization.
Recommended attribution: "We used the SBS Roche Transfluor image set provided by Ilya Ravkin and available from the Broad Bioimage Benchmark Collection (www.broad.mit.edu/bbbc)."
Analysis of the data set can be downloaded from http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html

本图像集展示了Transfluor检测实验，其中孤儿G蛋白偶联受体（G-protein coupled receptor，GPCR）被稳定地整合至表达GFP（绿色）的β-arrestin的U2OS细胞系中，同时呈现细胞核（红色）。在化合物与细胞共同孵育一小时后，使用固定剂（甲醛）终止反应，随后在Cellomics ArrayScan HCS Reader上使用GPCR生物应用读取板上的数据。阴性结果表现为细胞质中GFP分布更加均匀，而阳性结果则显示GFP在细胞核周围的定位。推荐引用：'我们使用了Ilya Ravkin提供并由Broad Bioimage Benchmark Collection（www.broad.mit.edu/bbbc）提供的SBS Roche Transfluor图像集。数据集分析可从http://www.broadinstitute.org/bbbc/sbs_roche_transfluor.html下载。'