HMGA2 project, CRISPR screen
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下载链接：
https://www.ncbi.nlm.nih.gov/sra/SRP312590
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资源简介：
sgRNA whole genome library sequencing in OCI-AML5-Cas9 EKO library cells overexpressing HMGA-YFP or control YFP vectors. The goal of this experiment is to identify synthetic lethal and synthetic rescue sgRNAs with regard to HMGA2 overexpression in AML. Overall design: OCI-AML5-Cas9 EKO library cells were infected with HMGA-YFP or control YFP vectors for 3 days and then treated with DOX for 7 days to induce Cas9 expression. Cells were further expanded for 7 days before DNA extraction. ID sequences of the sgRNAs were amplified by PCR and sequenced.
创建时间：
2022-08-08
