10x genomics single cell sequencing of yak hair follicle

Hair follicles of the yak are in anagen and catagen , the hair follicles of healthy female yaks around 2 years old are collected for the preparation of single-cell suspension, the cells were sequenced by scRNA-seq on the 10x genome platform. A total of about 12000 single-cell transcriptome information were obtained. According to the reported marker genes, the main cell groups in anagen and catagen of yak were identified. Based on the analysis of pseudotime trajectory, the differentiation trajectory of epidermal cell lineage and dermal cell lineage during hair follicle development and the dynamic changes of genes during differentiation were described. Overall design: Single-cell RNA sequencing was performed on a single cell in the hair follicle anagen and catagen of Tianzhu white yak scapula skin. Single-cell RNA sequencing was performed on a single cell in the hair follicle telogen of Tianzhu white yak scapula skin.