Small intestinal crypt cell subsets and gene expression in sleep-deprived mice

The level of 5-HT in the intestine of sleep-deprived mice is increased, which induces oxidative stress of intestinal stem cells by acting on HTR4 receptor. Single-cell RNA sequencing was used to analyze the changes in intestinal cell subsets and HTR4 expression after sleep deprivation Overall design: Intestinal epithelial cells of mice before and after sleep deprivation were isolated and analyzed using scRNA-seq