In vivo protein-protein crosslinking in human lens through lys or protein N-terminus with Gln/Glu residues

In this study we identified protein-protein crosslinking through lys or protein N-terminus with Gln/Glu residues in aged human lens tissues. Such crosslinks could be reproduced experimentally by incubation of Glu- or Gln-containing peptides and their formation were consistent with an amino group attacking a glutarimide intermediate.