Single-Molecule Long-Read Sequencing of Avocado Provides Microsatellite Markers for Genetic Diversity in a Group of Avocado Germplasm

A total amount of 5 g of total RNA (equally mixed with all RNAs) was used as input into the Clontech SMARTer reaction. Size fractionation and selection (1-6 kb) were performed using the BluePippin Size Selection System (Sage Science, Beverly, MA). One SMRT bell libraries were constructed with the Pacific Biosciences DNA Template Prep Kit 2.0 and SMRT sequencing was then performed on the PacBio RSII instrument (Pacific Bioscience, Menlo Park, CA, USA)