Stable integrative transformation of Trypanosoma brucei that occurs exclusively by homologous recombination.
收藏PubMed Central1991-03-15 更新2026-05-16 收录
下载链接：
https://pmc.ncbi.nlm.nih.gov/articles/PMC51180/
下载链接
链接失效反馈官方服务：
资源简介：
A calmodulin-neomycin-resistance fusion gene was introduced into Trypanosoma brucei by electroporation, and stably transformed cell lines were obtained. In all of the transformants, the fusion gene had integrated into the host genome at the cognate locus, evidently by homologous recombination within flanking calmodulin DNA. This unusual observation distinguishes trypanosomes as the only eukaryote other than yeast known to undergo gene targeting in essentially 100% of the stable transformants. It should now be possible to systematically manipulate the trypanosome genome, directing predetermined mutations to virtually any chromosomal locus. IMAGES:
提供机构：
National Academy of Sciences
创建时间：
1991-03-15
