Human RAD54 inhibitor screen: summary of 158 compounds identified as 'hits'

This dataset, Human RAD54 inhibitor screen: summary of 158 compounds identified as 'hits', accompanies the manuscript, Small molecule inhibitors of a human recombination-associated ATPase, RAD54. 158 compounds identified as inhibitory to the ATPase activity of purified human RAD54 on dsDNA (pUC19) in a primary malachite green phosphate detection assay are catalogued in an Excel document, with annotation describing follow-up in secondary assays (including IC50 values, DNA dependence of ATPase inhibition & class assignments, TopoI inhibition, structural similarity to known DNA intercalators, SF50 (toxicity to HEK293), combination index (CI) with mitomycin C (MMC) or cisplatin (CsPt), reversibility/irreversibility of RAD54 inhibition, PAINS classifications).Â本数据集为《人类RAD54抑制剂筛选：158个命中化合物汇总》，随论文《人类重组相关ATP酶RAD54的小分子抑制剂》一同发布。本数据集收录了158个经初步孔雀石绿磷酸盐检测试验（malachite green phosphate detection assay）验证，可抑制纯化人类RAD54在双链DNA（double-stranded DNA, dsDNA，pUC19）底物上ATP酶活性的化合物，相关信息整理于一份Excel文档中。文档附带注释，详细描述了该批化合物在二级验证试验中的后续表征数据，包括半抑制浓度（IC50）、ATP酶抑制作用的DNA依赖性及类别划分、拓扑异构酶I（Topoisomerase I, TopoI）抑制活性、与已知DNA嵌入剂的结构相似性、对HEK293细胞毒性相关的SF50值、与丝裂霉素C（mitomycin C, MMC）或顺铂（cisplatin, CsPt）联用的联合指数（CI）、RAD54抑制作用的可逆性与不可逆性，以及泛化验干扰化合物（Pan-Assay Interference Compounds, PAINS）分类。