Faecal 1H NMR spectroscopy data from medically healthy dogs and dogs suffering from cutaneous renal glomerular vasculopathy (CRGV)

Canine Cutaneous and Renal Glomerular Vasculopathy (CRGV) is a fatal idiopathic disease that has resulted in the death of ~ 330 dogs in the UK since 2012. The disease is characterised by cutaneous lesions/ulcerations on the distal extremities, vasculitis of the glomerular arteries and acute kidney injury. Here we characterised the faecal metabolome of CRGV-affected dogs and medically healthy (MH) dogs using proton nuclear magnetic resonance spectroscopy. A total of 0.2 g of faeces from CRGV-affected (n = 31) and MH (n = 48) dogs, homogenised, and vortexed until no visible particles could be observed. The samples were run on a 700 MHz Bruker NMR spectrometer equipped with a cryoprobe and a SampleJet autosampler maintained at 4°C. A principal component analysis revealed distinct clustering of MH samples, while CRGV-affected samples were more distributed across the scores plot. In addition, an orthogonal partial least squares discriminant analysis (Q2Y = 0.24; p = 0.01) revealed distinct differences in the metabolite profiles between cohorts, with several metabolites associated with early-onset starvation (choline, valine, alanine, methylsuccinic acid) being highly represented in CRGV-affected dogs.