The 5-AA deletion does not significantly affect the ID<sub>50</sub> value in immunodeficient mice <sup>a</sup>.
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aThe ΔospC/FL/1, ΔospC/FL/2, ΔospC/ΔNt5/1 and ΔospC/ΔNt5/2 spirochetes were grown to late-log phase (108 cells per ml) and 10-fold serially diluted with BSK-H medium. Approximately 100 µl of bacterial suspension was intradermally/subcutaneously inoculated into each C3H SCID mouse. Animals were sacrificed 1 month later; heart, tibiotarsal joint and skin specimens were harvested for bacterial isolation. The ID50 values were calculated by the method of Reed and Muench [22].
创建时间：
2010-12-31
