Sulphate reduction and methane oxidation rates of Håkon Mosby mud volcano sediments measured at station MSM16/2_838-1 (Sample 3, new flow surface)@en

Sulfate reduction (SR) and anaerobic oxidation of methane (AOM) were measured ex situ by the whole core injection method (doi:10.1080/01490457809377722). We incubated the samples at in situ temperature (1.0°C) for 12 hours with either 14** CH4 (dissolved in water, 2.5 kBq) or carrier-free 35** SO4 (dissolved in water, 50 kBq). Sediment was fixed in 25 ml 2.5% sodium hydroxide (NaOH) solution or 20 ml 20% ZnAc solution for AOM or SR, respectively. Turnover rates were measured as previously described (http://edoc.mpg.de/177065; doi:10.4319/lom.2004.2.171).本研究通过全岩心注入法（whole core injection method）对硫酸盐还原作用（Sulfate reduction, SR）与甲烷厌氧氧化作用（anaerobic oxidation of methane, AOM）进行异位（ex situ）测定，相关方法的文献DOI为10.1080/01490457809377722。实验将沉积物样品于原位温度（1.0℃）下孵育12小时，孵育体系中分别加入溶于水的14**标记甲烷（活度2.5 kBq）或无载体35**标记硫酸盐（活度50 kBq）。用于甲烷厌氧氧化作用与硫酸盐还原作用测定的沉积物样品，分别采用25 mL 2.5%氢氧化钠（sodium hydroxide, NaOH）溶液与20 mL 20%乙酸锌（zinc acetate, ZnAc）溶液进行固定。周转速率的测定参照此前已发表的方法完成，相关参考链接与DOI分别为http://edoc.mpg.de/177065与10.4319/lom.2004.2.171。