Bacillus subtilis in the treatment of banana plantlets infected by nematodes

ABSTRACT The use of healthy propagative plant material is an important management method for nematodes in banana crops (Musa spp.), once it prevents the introduction of nematodes in non-infested areas. The aim of this work was to evaluate the effect of the bio-nematicide Nemathel® (Bacillus subtilis) on Terra banana plantlets cv. Comprida infected with a mixed population of Radopholus similis, Meloidogyne spp., Pratylenchus spp., and Helicotylenchus spp. Different doses of Nemathel® (50, 100, 150, 200, and 250 mL/10 L water) were tested in addition to the positive (nematicide) and negative (water) treatments. The plantlets were immersed in each treatment for 30 min and subsequently, planted in pots containing sterilized soil in greenhouse. The evaluation was performed four months later. Dosages of 200 and 250 mL of Nemathel®/10 L in water showed the greatest reduction of nematode populations in roots and rhizomes.摘要 采用健康繁殖植物材料是防治香蕉作物（Musa spp.，芭蕉属）线虫病害的重要管理手段，可有效阻止线虫传入未侵染区域。本研究旨在评估生防杀线虫剂（bio-nematicide）Nemathel®（枯草芽孢杆菌，Bacillus subtilis）对混合侵染了相似穿孔线虫（Radopholus similis）、根结线虫属（Meloidogyne spp.）、短体线虫属（Pratylenchus spp.）以及盘旋线虫属（Helicotylenchus spp.）的‘Comprida’品种泰拉香蕉幼苗的防治效果。实验设置了50、100、150、200及250 mL/10 L水共5种梯度的Nemathel®药剂浓度，并设置阳性对照（化学杀线虫剂）与阴性对照（清水）。将香蕉幼苗分别浸入各处理液中30分钟，随后移栽至装有灭菌土壤的盆钵中，置于温室培育。于移栽四个月后开展线虫种群检测。结果显示，200 mL/10 L水与250 mL/10 L水两个浓度的Nemathel®可最大程度降低香蕉根系与根状茎中的线虫种群数量。