Increases in fluorescence intensity for different donor fluorescent proteins fused to Ultramarine after protease cleavage.
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1Increase in fluorescence emission was monitored until an endpoint was reached. Complete cleavage of the polypeptide linker was confirmed by subjecting endpoint samples to analysis SDS-PAGE. 2R0 values were calculated according to [29]. For comparison data is included for the fusion protein ECFPC3EYFP, a probe reported for measuring caspase 3 protease activation in cells [30].
创建时间：
2013-02-20
