Process and microbial analysis of AD systems

Raw data on fermentation parameters
(biogas, methane %, alkalinity, pH) and PLFA and PLEL analysis are included
into the excel spreadsheets: “First OLR_Fermentation + PLFA and PLEL” and
“Second OLR_Fermentation + PLFA and PLEL”. Fermentation data are related to
mesophilic control digesters running at 1.4 kgVS/m3d and upset digesters
running at 2.9 and 3.9 kgVS/m3d (medium and high.).

The letters: a, b, c, d, e, f, g, h and i
represent the triplicates of control and the upset systems where:

a, b and c = control
reactors;

d, e and f = reactors with increased OLR to 2.9 kgVS/m3d (sometimes
referred as medium); and

g, h, and i = reactors
with increased OLR to 3.9 kgVS/m3d (sometimes referred as high)

Analysis of the data and graphs included
in the paper “Organic loading rate: a promising microbial management tool in anaerobic
digestion “ are included in the files “data for graphs’, the data used can be visualised
clicking on the curve of interest.

Raw pyrosequencing data is included in the
zip files and analysis of the data in the separate file “pyrosequencing data
analysis”

Primers design has been included into the
“Primers” file.