Measuring E-Cadherin Expression In Nsd3Short Overexpressing H1299 Cells

<strong>SGC Open Notebook Project to Characterize the HMTase NSD3</strong> <strong>Exp021 Objective:</strong> During epithelial to mesenchymal transitioning (EMT), transcription networks become activated and cytoskeletal rearrangements take place. These events promote migratory capacity and invasiveness. Cadherins are a family of cell adhesion molecules central in transitioning to a more mesenchymal state. E-cadherin, a marker for polarized epithelial cells, is downregulated during EMT. Here, I investigate the effect of NSD3short overexpression on E-cadherin expression in H1299 cells, which would support NSD3 as a EMT driver.