Genome-wide analysis of coding and non-coding RNAs in ovary of honey bee workers

The present study is the first study to identify the involvement of mRNA, lncRNAs, circRNAs and miRNA in the ovary of honey-bee workers.We predicted 10271 mRNAs, 7235 lncRNAs, 11794 circRNAs and 164 miRNAs in the ovary of honey bee workers. Overall design: To identify the involvement of mRNAs, lncRNAs, circRNAs and miRNAs in the ovary of honey-bee workers, Pools of honey bee workers' ovaries were used in this study (N=3). Ovaries of worker bees sampling were carried out according to the procedure of Macedo, with minor modifications (Macedo et al. 2016). Briefly, 5 colonies with sister queens were reared using standard beekeeping techniques (Harbo 1986). The frames with emerging broods from these colonies were placed in an incubator (34 ? and 80% relative humidity). Newly emerged workers were paint marked and returned to their original colony. After 4 days, the marked workers were collected. Their ovaries were dissected, snap frozen in liquid nitrogen and stored at -80 ?. For each biological sample, 40 ovary pairs were dissected from workers of one single colony. Totally, three samples were used for RNAseq.