Genome sequencing of Kummerowia stipulacea

For whole-genome DNA sequencing, a modified CTAB method was employed for the extraction of total genomic DNA (Doyle and Doyle 1987). The construction of paired-end libraries, characterised by an insertion size of 350 base pairs, was undertaken in accordance with the manufacturer's instructions. The subsequent process of sequencing was performed on an DNBSEQ-T7 platform (MGI Tech Co., Ltd., Shenzhen, China), resulting in a total of 7.24 Gb Illumina reads