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Pol IV and RDR2-dependent precursors of 24 nt siRNAs. Arabidopsis thaliana cultivar:Columbia-0

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NIAID Data Ecosystem2026-03-08 收录
下载链接:
https://www.ncbi.nlm.nih.gov/bioproject/PRJNA287854
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资源简介:
In Arabidopsis thaliana, 24 nt siRNAs guide cytosine methylation to complementary DNA sequences, helping silence transposons and a subset of genes. Biogenesis of 24 nt siRNAs requires nuclear multisubunit RNA Polymerase IV (Pol IV), RNA-DEPENDENT RNA POLYMERASE 2 (RDR2) and DICER-LIKE3 (DCL3). We used Illumina deep sequencing of 15-80 nt RNAs from wild type (Col-0) and dcl2 dcl3 dcl4 triple mutant plants to identify short dsRNAs that are synthesized by the Pol IV-RDR2 complex, and which are subsequently diced by DCL3.
创建时间:
2015-06-23
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