Data supporting development and validation of liquid chromatography tandem mass spectrometry method for the quantitative determination of bile acids in feces

The data presented here support the optimization of feces sample extraction for sensitive quantification of bile acids in human feces using LC-MS/MS. The dataset includes: Comparison between recovery of bile acids from (a) dried feces, (b) dried feces spiked with deuterated internal standards prior to drying, and (c) wet feces Short-term stability data for bile acids standard solutions and fecal extracts containing bile acids after 24 h storage at room temperature and at 6°C Data for multiple freeze-thaw cycles on bile acids recovery in both standard solutions and fecal samples Bile acids recovery from cold and warm extractions data本数据集旨在支撑采用液相色谱-串联质谱（LC-MS/MS）对人体粪便中胆汁酸进行高灵敏定量分析的粪便样品萃取方法优化研究。 数据集包含以下内容： 1. 三种不同处理方式下粪便中胆汁酸回收率的对比数据：(a) 干燥粪便样品；(b) 干燥前添加氘代内标的干燥粪便样品；(c) 湿粪便样品； 2. 胆汁酸标准溶液及含胆汁酸的粪便提取物分别在室温与6℃条件下储存24小时后的短期稳定性数据； 3. 标准溶液与粪便样品经多次冻融循环后对胆汁酸回收率影响的相关数据； 4. 冷萃取与热萃取两种方式下的胆汁酸回收率数据。