Lipidomics Methods Lipid extraction: HEK293T cells were seeded in 6-well plates, grown to 70% confluency and treated with bumped fatty acid probes (15 µM) for 4 h. Cells were dislodged i
Cell membranes insulate and mediate interactions between life and its environment, with lipids determining their properties and functions. However, the intricacies of how cells adjust their lipidome c
Toll-like receptor 4 (TLR4)-mediated changes in macrophages reshape intracellular lipid pools to coordinate an effective innate immune response. Although this has been previously well-studied in diffe
Direct infusion mass spectrometry serum phosphatidylcholine acyl-alkyls (PC ae) (μM) from 14 lean (L-MAINT) and 16 obese (O-MAINT) cats during weight maintenance and after 10-week energy restriction (
