Data from: Isolation and characterization of 145 polymorphic microsatellite loci for the common frog (Rana temporaria)

We describe primers and polymerase chain reaction conditions to amplify 145 di-, tri- and tetranucleotide microsatellite loci from the common frog (Rana temporaria), a species commonly used as a model in ecological and evolutionary research. Primers were tested on 46 individuals from two Fennoscandian populations and yielded an average of six to nine alleles per locus (range = 1–30) depending on the population. Average observed heterozygosities in the two populations were 0.16 (range = 0–0.91) and 0.36 (range = 0–1).本研究描述了用于从普通蛙（Rana temporaria）中扩增145个二核苷酸、三核苷酸及四核苷酸微卫星位点（microsatellite loci）的引物及聚合酶链式反应（polymerase chain reaction）条件；该物种常被用作生态与进化研究的模式生物。研究人员对来自两个芬诺斯坎底亚种群的46个个体进行了引物测试，结果显示每个位点的等位基因数平均为6至9个（范围1~30），具体数值因种群而异。两个种群的平均观测杂合度分别为0.16（范围0~0.91）与0.36（范围0~1）。