m6Am RNA methylation enzyme PCIF1 restricts HIV infection and is degraded by viral Vpr protein. m6Am RNA methylation enzyme PCIF1 restricts HIV infection and is degraded by viral Vpr protein

To identify m6Am genes that are responsible for HIV inhibition, MeRIP-seq was performed in control and PCIF1 KO T cells infected with HIV. Overall design: Control or PCIF1 KO Jurkat cells were mock treated or infected with HIV LAI strain at an MOI of 4 for 3 days. Change of RNA methylation states was detected by MeRIP-seq. m6Am genes were identified as 5'UTR methylated peaks that are depleted in PCIF1 cells