Concentrations of DTDA that induces 50% inhibition in TcTIM and some of its mutants.

The effect of DTDA on the indicated enzymes was determined as in Figure 1, from the data the concentration that induced half-maximal inhibition was calculated. NE indicates that 200 µM DTDA had no effect.*As noted in the text, relatively low DTDA concentrations inhibit the activity by approximately 50%; significantly higher concentrations were required for full inhibition. The data in the Table indicate the concentration at which half-maximal inhibition of the initial phase was achieved.